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DOT · Drivers & safety-sensitive employees

DOT drug & alcohol testing, done by the book.

Federal DOT drug and alcohol testing for drivers and safety-sensitive employees — collected under 49 CFR Part 40, processed by SAMHSA-certified labs, and reviewed by a Medical Review Officer. Pre-employment, random, post-accident, and reasonable suspicion, all handled locally and fast.

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FMCSA-Certified ExaminersDATIA Member StandardsSAMHSA-Certified LabsSame-Day & Walk-InChain-of-CustodyCourt & DOT Compliant

The DOT testing categories we cover

  • Pre-employment — required before a driver performs safety-sensitive duties
  • Random — unannounced selections through your consortium or company program
  • Post-accident — after qualifying crashes, on the strict DOT timeline
  • Reasonable suspicion — when trained supervisors document signs of use
  • Return-to-duty & follow-up — after the SAP process

What's on the DOT panel in 2026

The DOT urine panel tests for five categories: marijuana (THC), cocaine, amphetamines (including methamphetamine and MDMA), opioids, and PCP. As of 2025 the opioid group includes semi-synthetic opioids, and fentanyl has been added to federal panels. DOT breath and, where authorized, oral-fluid alcohol testing is also available. Every non-negative result is reviewed by an independent Medical Review Officer before it's reported.

Employers: we set your DOT and non-DOT programs up on one standing account so pre-employment, randoms and post-accident events are all handled without you chasing collectors. See employer accounts.

Chain-of-custody & MRO review

DOT testing is strict for a reason. Every collection uses the federal chain-of-custody form (CCF), tamper-evident seals, and split-specimen handling so a driver can request a re-test of the split if needed. Results go to a certified lab and then to a Medical Review Officer, who verifies any positive against legitimate medical explanations before reporting. We follow the process exactly so your results are compliant and defensible.

Clearinghouse-aware

For FMCSA-regulated drivers, pre-employment queries and violations run through the DOT Drug & Alcohol Clearinghouse. We keep your program aligned with Clearinghouse requirements so nothing slips through the cracks.

Answers

Frequently asked — DOT Drug & Alcohol Testing

What drugs does a DOT drug test screen for?

The DOT 5-panel screens for marijuana, cocaine, amphetamines/methamphetamine, opioids (including semi-synthetics and fentanyl on updated panels), and PCP. All positives are MRO-reviewed.

How fast are DOT results?

Negative results are often reported within 24–48 hours. Non-negatives take a bit longer because they require lab confirmation and Medical Review Officer review.

Can you handle post-accident and reasonable-suspicion testing?

Yes. Post-accident testing has strict DOT time limits — come straight in and we collect right away under chain-of-custody, with reasonable-suspicion testing available the same day.

What's the difference between DOT and non-DOT testing?

DOT testing follows federal 49 CFR Part 40 procedures with a set panel and MRO review. Non-DOT (employer) testing is more flexible — you choose the panel and policy. We do both.

Do you handle the FMCSA Clearinghouse?

We keep your program aligned with Clearinghouse requirements, including pre-employment query awareness and reporting expectations for regulated drivers.

Fresno · Clovis · Madera · The Central Valley

Need a test today? Talk to a real person.

Walk-ins welcome and same-day appointments across the Central Valley. Call or text now and we'll get you scheduled fast — court-ordered, DOT, employer, or individual. Results handled with full chain-of-custody.

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